Tag

Part:BBa_K1381024:Design

Designed by: Viktor Blomkvist, Tim Hagelby Edstrom, Laura Pacoste and Tersea Reinli   Group: iGEM14_Uppsala   (2014-10-07)


B0034-PgsA-(EAAAR)x4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1219
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1149


Design Notes

Designed with freiburg fusion standard but ony got it to work by using normal E, X, S, P restriction


Source

PgsA gene from Bacillus Subtilis

References